The cytoplasmic cytoskeleton determines cell organization, shape and adhesion among other functions.
Furthermore, the cell type-specific expression of intermediate filaments allows determination of the origin of many, otherwise unspecific tumors.
Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation, Immunocytochemistry and Western Blot.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry: 1:250- 1:1600Immunoprecipitation: 1:10Western Blot: 1:1000-1:2500Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：50ug
Host：rabbit
Grade：Affinity Purified
Purity：Purified by Immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, 1mg/ml BSA, 0.01% sodium azide.
Specificity：Recognizes human actinin-4. Does not cross-react with actinin-1, -2 or -3. Species Crossreactivity: mouse, rat, porcine and Chinese hamster.
Isotype：IgG
Calc Applications Abbrev：IC IHC IP WB
Calc Crossreactivity：Hm Hu Mo Po Rt
Immunogen：Synthetic peptide corresponding to aa27-38 of the N-terminal α-Actinin-4, coupled via a C-terminal cysteine residue to KLH.
