DR5, also known as TRAIL-R2, TRICK2 and KILLER, is a widely expressed type I membrane member of the tumor necrosis (TNF) receptor family.
Members of this family contain multiple cysteine-rich repeats in their extracellular domain and while the cytoplasmic region is less conserved, they do contain an intracellular 80 amino acid motif known as the ‘death domain’ (DD).1, 2, 3, 4 DR5 is a receptor for the cytotoxic ligand TRAIL which induces apoptosis and it is the cytoplasmic DD that is responsible for transducing the death process.5, 6 DR5 has also been shown to activate NF-kappaB.7
Applications:
Flow cytometry
Western Blot
Induction of apoptosis
Recommended Dilution:Western Blot: 1-2ug/mlFlow Cytometry: ≤ 3ug/10e6 cellsInduction of Apoptosis: 2.5ug/ml for 12 hours (10-12% in Jurkat cells)Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%).
Store at -20°C or colder.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in 100mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added.
