Zeta-associated protein (ZAP-70), a 70kD member of the Syk tyrosine kinase family, plays a central role in lymphocyte activation and development and is implicated in several immune disorders.
Upon T-cell antigen receptor (TCR) engagement, ZAP-70 is phosphorylated on tyrosines 292, 315 and 319 in the interdomain B, located between the SH2 and kinase domains.
Phosphorylation of tyrosine 315 and 319 enhances ZAP-70 function in mediating lymphocyte signaling, while tyrosine 292 terminates transient activation and attentuates lymphocyte signaling.
ZAP-70 tyrosine 292 is the negative regulatory site that modulates the duration of activated TCR at the cell surface and functions as a docking site for Cbl
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells treated with H2O2.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human ZAP-70 when phosphorylated at Tyr292.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human ZAP-70 that contains tyrosine 292. Species sequence homology: mouse 100%.
