84-1078-03　Vav1, phosphorylated (Tyr160) (GDP/GTP Exchange Factor) 10Blots　V2121-07A

特徴

• Vav proteins belong to the guanidine nucleotide exchange factor family of proteins that couple receptors to Rho-GTPases.
• The Vav family has currently three members in mammalian cells (Vav1, Vav2, and Vav3).
• Vav1 contains multiple protein binding motifs, indicating a role for Vav1as an adaptor protein linking cell surface receptors to downstream signaling proteins.
• Vav1 is exclusively expressed in hematopoietic cells and plays a significant role in lymphocyte development and antigen receptor mediated activation of NFAT and NFkB, and in more delayed responses such as the production of cytokines, including IL-2, IL-3, IFN-y, and TNF-a.
• Vav1 is also reported to activate JNK, p21-activated kinase (PAK) and PLCy, implicating its critical role in calcium mobilization, and cytoskeletal rearrangement.
• Activation of several cell surface receptors including integrins, immune response receptors, or growth factor receptors leads to phosphorylation of Vav1 on tyrosine residues including tyrosine 160.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:K562 cells expressing wild-type human Integrin av and b3 proteins
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.