84-1078-01　［取扱停止］Vascular Endothelial Growth Factor Receptor 2, phosphorylated (Tyr1054, Tyr1059) (VEGF R2, Flk1, KDR, Receptor Tyrosine Kinase) 10Blots　V2110-19D

特徴

• Vascular Endothelial Growth Factor Receptor 2 (VEGFR2, also known as KDR/FLK-1) is a ~200kD member of a receptor tyrosine kinase family whose activation plays an essential role in a large number of biological processes such as embryonic development, wound healing, cell proliferation, migration and differentiation.
• Like other growth factor receptors upon ligand binding, VEGFR2 forms a dimer and is autophosphorylated on multiple tyrosine residues.
• These sites can be involved in the regulation of kinase activity or serve as binding sites for SH2- and phosphotyrosine binding domain containing signaling proteins.
• Phosphorylation of tyrosines 1054 and 1059 in the activation loop is required for activation of VEGFR2 and its intrinsic tyrosine kinase activity.
• Applications:Suitable for use in Western blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilution to be determined by researcher.
• Positive Controls:Porcine aortic endothelial (PAE) cells transfected with a chimeric receptor consisting of the extracellular portion of the CSF-1 receptor fused with the transmembrane and intracellular domains of mouse VEGFR2.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.