Phosphorylation site-specific antibody selective for the phosphorylated form of human tau containing a phosphate on serine 422.
Tau is a neuronal microtubule-associated protein found predominantly on axons.
The function of tau is to promote tubulin polymerization and stabilize microtubules, but it also serves to link certain signaling pathways to the cytoskeleton.
Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions seen in Alzheimer’s disease (AD).
Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to degeneration of the affected neurons.
Hyperphosphorylated tau is also found in a range of other central nervous system disorders.
Numerous serine/threonine kinases, including GSK-3b, PKA, CDK5, MARK, MAPKs, and casein kinase II can phosphorylate tau.
Serine 422 can be phosphorylated by members of the MAP kinase (MAPK) family.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by sequential epitope-specific chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes human Tau. Species sequence homology: mouse and rat.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human tau that contains serine 422.
