Tau is a neuronal microtubule-associated protein found predominantly on axons.
The function of tau is to promote tubulin polymerization and stabilize microtubules.
Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain.
Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders.
Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons.
Numerous threonine/threonine kinases, including GSK-3b, protein kinase A (PKA), cyclin-dependent kinase 5 and casein kinase II, phosphorylate tau.
Threonine 212 is phosphorylated by GSK-3b, cdk5 and PKA in vitro and in vivo and has been shown to be specific for processes found in neurodegenerative diseases.
Applications:Suitable for use in Western Blot, Flow Cytometry.
Other applications have not been tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilution to be determined by the researcher.
Positive Control:Recombinant human tau
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG and protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Tau when phosphorylated at Thr212.
Isotype：IgG
Calc Applications Abbrev：FC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human tau that contains threonine 212. Species Sequence Homology: mouse, rat, Rhesus monkey, baboon, bovine and goat.
