84-1077-87　［取扱停止］Tau, phosphorylated (Ser199) (Tau Protein, Microtubule-associated Protein, MAP) 10Blots　T1032-51

特徴

• Tau is a neuronal microtubule-associated protein found predominantly on axons that functions to promote tubulin polymerization and stabilize microtubules.
• Tau, in its hyperphosphorylated form, is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer’s disease (AD) brain.
• Hyperphosphorylated tau is also found in neurofibrillary lesions in a range of other central nervous system disorders.
• Hyperphosphorylation impairs the microtubule binding function of tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons.
• Numerous serine/threonine kinases including GSK-3 , protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II, phosphorylate tau.
• Phosphorylation of serine 199 is catalyzed by GSK-3 in vitro and has been linked to frontotemporal dementia.
• Applications:Suitable for use in Western Blot.
• Other applications have not been tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilution to be determined by the researcher.
• Positive Control:Recombinant human Tau treated with GSK-3 ; African green monkey kidney (CV-1) cells, stably expressing human four repeat tau and SV40 small T antigen to induce specific inhibition of PP2A.
• Storage and Stability:May be stored at 4°C for short-term only.
• For long-term storage, store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.