Syk (72kD) is a non-receptor protein tyrosine kinase that plays a central role in immune receptor signal transduction and is implicated in endothelial cell functions, including cell growth and migration.
Syk is activated upon binding to immunoreceptor tyrosine-based activating motif (ITAM) and involved in coupling activated immunoreceptors to downstream signaling.
Syk undergoes phosphorylation on multiple tyrosines within the linker region that separates the Cterminal catalytic domain from the N-terminal SH2 domains (tyrosines 323, 348 and 352 or 317, 342 and 346 using the mouse sequence).
The phosphorylation of Syk tyrosine 323 – a Cbl binding site – mediates Cbl-induced negative regulation of Syk.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilution to be determined by the researcher.
Positive Control:Jurkat cells treated with H2O2
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Syk when phosphorylated at Tyr323. Species sequence homology: Mouse Syk phosphorylated at Tyr317 (79%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human Syk that contains tyrosine 323 (tyrosine 317 in the mouse sequence).
