Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors.
Tyrosine 418 is located in the catalytic SH2 domain and is one of the autophosphorylation sites.
Full catalytic activity of Src requires phosphorylation of tyrosine 418.
This activity is negatively regulated when tyrosine 529 of Src is phosphorylated by Csk.
This conformation holds Src in the inactive form through an intramolecular interaction between the SH2 domain and the carboxyl terminus.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilution to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Src when phosphorylated at Tyr418. Species crossreactivity: mouse, and chicken. Species sequence homology: Other species (100%). Also reacts with Fyn and Yes.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Ch Hu Mo
Immunogen：Synthetic phosphopeptide corresponding to human Src that contains tyrosine 418 (tyrosine 419 including the initiating methionine).
