Src (also known as pp60src) is a non-receptor tyrosine kinase involved in signal transduction in many biological systems and implicated in the development of human tumors.
This kinase is expressed in different tissues of the body with the highest protein levels detected in neurons and platelets.
Src can modulate the signal transduction pathways activated by several growth factors (e.g., PDGF, M-CSF and G-CSF) and integrins.
Src also regulates the activity of several ion channels including the N-methyl-D-aspartate (NMDA) receptor.
In addition, Src is thought to play a role in physiological/pathophysiological processes in the central nervous system.
This antibody is useful to determine total levels of Src protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:CEF cell extracts +/- Src; human platelets
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ug
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human and mouse Src. Species sequence homology: Rat (100%) and chicken Src (93%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to aa6-19 from human Src.
