The Src family of non-receptor tyrosine kinases is involved in the modulation of a wide variety of normal and pathogenic cellular processes including growth, development, differentiation and carcinogenesis.
These proteins contain a consensus structure comprised of one or more N-terminal SH2 and/or SH3 domains involved in protein-protein interactions, a central catalytic tyrosine kinase domain and a short C-terminal domain.
This C-terminal domain contains a tyrosine residue that, when phosphorylated by Csk tyrosine kinase, functions as a negative regulatory site (tyrosines 530, 531, 522, 505 and 508 in Src, Fyn, Hck, Lck and Lyn, respectively) by providing a binding site for the N-terminal SH2 domain.
The association of these two domains blocks access to acritical tyrosine residue in the activation loop, the phosphorylation of which is required for enzyme activation.
When the C-terminal domain tyrosine residue is dephosphorylated, the catalytic domain tyrosine can be maximally phosphorylated leading to full kinase activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Positive Control:PC12 cells +/- sorbitol, Chick Embryofibronectin and recombinant activated Fyn, Lck and Lyn proteins
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human Src kinase family members (Src, Fyn, Lck, Lyn, and Hck). Species sequence homology: mouse and rat, and also among other members of the Src protein family.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to human Hck that contains tyrosine 522. Species sequence homology: mouse and rat, and also among other members of the Src protein family.
