Smad3 is a ~50kD member of a family of proteins that act as key mediators of TGF- superfamily signaling in cell proliferation, differentiation and development.
The Smad family is divided into three subclasses: receptor-regulated Smads, activin/TGF- receptor-regulated (Smad2 and 3) or BMP receptor regulated (Smad 1, 5 and 8); the common partner, (Smad4) that functions via its interaction to the various Smads; and the inhibitory Smads, (Smad6 and 7).
Activated Smad3 oligomerizes with Smad4 upon TGF- stimulation and translocates as a complex into the nucleus, allowing its binding to DNA and transcription factors.
Phosphorylation of the two TGF- -dependent serines 423 and 425 in the C-terminus of Smad3 is critical for Smad3 transcriptional activity and TGF- signaling.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:NMuMG cells +/- TGF-
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Smad3 when phosphorylated at Ser423 and Ser425. Species sequence homology: Mouse (100%). Does not cross-react with Smad2.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human Smad3 that contains serine 423 and serine 425.
