SHP2 (SH2-domain containing tyrosine phosphatase 2, also known as SHPTP2) is a ~70kD tyrosine phosphatase that plays an important role in cell growth and transformation and mediating integrin, Src and receptor tyrosine kinase (RTK) signaling.
SHP2 undergoes tyrosine phosphorylation on several sites including tyrosines 542 and 580 upon binding to platelet-derived growth factor receptor beta (PDGFRb), in response to PDGF stimulation.
Tyrosine 542 mediates SHP2 binding to the SH2 domain of Grb2, which in turn couples RTKs to Ras.
Phosphorylated tyrosine 542 binds intramolecularly to the SH2 domain in the NH2 terminus of SHP2 and relieves phosphatase inhibition and mediates SHP2 activation of MAPK kinase pathways.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:NIH3T3 cells treated with PDGF
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes mouse SHP2 when phosphorylated at Tyr542. Species sequence homology: Human and rat (100%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic phosphopeptide corresponding to human SHP2 that contains tyrosine 542. Species sequence homology: Mouse, rat and chicken.
