Shc is a signaling and transforming protein that binds Src homology 2 and 3 (SH2 and SH3) domains.
Shc mediates the signaling from many types of receptors, such as those for growth factors, antigens, cytokines, integrins and G-protein coupled receptors, to their downstream proteins.
This downstream signaling occurs via binding to Grb2, an adaptor protein for the Ras exchange factor Sos, leading to activation of the Ras/Raf/MAPK pathway.
There are three isoforms of Shc (p46, p52 and p66), where p46 and p52 are the most widely expressed.
Phosphorylation of Shc at tyrosines 239, 240 and 317 potentiates its function as a regulatory adaptor protein.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:A431 cells +/- EGF
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human Shc when phosphorylated when Tyr239 at Tyr240. Mouse Shc has not been tested. Cross-reacts with insulin receptor in CHO cells over-expressing insulin receptor.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human Shc that contains tyrosines 239 and 240. Species sequence homology: mouse.
