Target Group: Rb is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F-1, PU.1, ATF-2, UBF, Elf-1 and c-abl.
This ability to alter transcription is regulated by phosphorylation catalyzed by the cyclin-dependent protein kinases (cdks).
Rb contains at least 16 consensus sequences for cdk phosphorylation, but the significance of all of these sites is unclear.
Phosphorylation of threonine 356 is catalyzed by the cdk4 complex Cyclin D-cdk4 and plays a role in mediating growth suppression activity of Rb.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.25-0.75ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells in high growth phase.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.05% sodium azide.
Specificity：Recognizes human Rb when phosphorylated at Thr356.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human Rb that contains threonine 356 (P06400). Species sequence homology: mouse (80%) rat (87%).
