Rb is a 110kD tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F-1, PU.1, ATF-2, UBF, Elf-1 and c-Abl.
This ability to alter transcription is regulated by phosphorylation catalyzed by the cyclin-dependent protein kinases (cdks).
Phosphorylation of serine 249 and threonine 252 is catalyzed by Cyclin Dcdk4.
Rb contains at least 16 consensus sequences for cdk phosphorylation, but the significance of many of these sites is unclear.
Phosphorylation by cdk4 appears to prime Rb for its subsequent phosphorylation by cdk2 and inhibits HDAC binding, DNA acetylation and Rb active repression.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells in high growth phase.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Rb when phosphorylated at Ser249 andThr252. Species sequence homology: Mouse and rat (100%), chicken (92%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human Rb that contains serine 249 and threonine 252 (P06400).
