Rac, cdc42 and Rho A, B and C are members of a small RhoGTPase family that bind and hydrolyze GTP.
GTP-bound Rac1 and cdc42 play a pivotal role in controlling cell shape, adhesion, growth and transformation.
Active Rac1 is implicated in regulating serum response element (SRE), NFAT1 and nuclear factor kB (NF-kB) transcription activities.
Activated Rac1 and cdc42 bind and activate PAK1, which in turn activates key downstream signaling proteins including MEKK-1 and JNK.
Rac1 and cdc42 are phosphorylated on serine 71, a putative Akt site located between the proteinbinding domain and GTP-binding domain.
Phosphorylation of Rac1 on serine 71 regulates its GTP-binding and GTPase activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000.
Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by affinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Rac1 when phosphorylated at Ser71. Species sequence homology: mouse, 100%. Cdc42 [pS71], 100% and RhoA/B/C [pS73], 92% are also expected to react.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from a region of human Rac1-protein that contains serine 71.
