Proline-rich/Ca2+-activated tyrosine kinase (Pyk2) is a member of the FAK family of non-receptor, proline-rich PTK's.
Pyk2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines and certain stress stimuli.
Pyk2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death.
Phosphorylation of the catalytic domain activation loop tyrosines 579 and 580 by Src results in maximum activation of Pyk2.
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Chicken embryo fibroblasts expressing human Pyk2 protein and plated on fibronectin
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Pyk2 when phosphorylated at Tyr579. Species sequence homology: Mouse and rat (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human Pyk2 that contains tyrosine 579. Species sequence homology: mouse and rat.
