Proline-rich/Ca2+-activated tyrosine kinase (Pyk2) is a member of the FAK family of non-receptor, proline-rich protein tyrosine kinases.
Pyk2 signaling is initiated by a variety of extracellular stimuli including integrin ligation, CD surface marker ligation (e.g., CD3, CD28, TCR, VCAM), bioactive peptides, growth factors, cytokines, chemokines and certain stress stimuli (reactive oxygen species and Ca++ flux).
Pyk2 is involved in the regulation of vesicular transport, osteoclastic bone resorption, modulation of ion channels, T- and B-cell receptor signaling and cell death.
Pyk2 tyrosine 402 is autophosphorylated, an important initial step for full activation of the kinase.
This phosphorylation also allows binding of Src and the p85 subunit of PI3-kinase.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Chick Embryo Fibroblast (CEF) cells expressing human Pyk2
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Pyk2 when phosphorylated at Tyr402. Species sequence homology: Rat and chicken Pyk2 (100%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to human Pyk2 that contains tyrosine 402. Species sequence homology: rat.
