PTEN (Phosphatase and tensin analog) is a ~58-60kD lipid phosphatase and tumor suppressor protein that is frequently mutated in tumor cells.
It plays a key role in cell migration, survival and apoptosis by negatively regulating phosphoproteins in the Akt/PI3Kinase pathway.
The noncatalytic regulatory carboxyl terminus of PTEN contains multiple phosphorylation sites including two CK2 sites (serines 370 and 385) and MAGI-2 sites (threonines 382 and 383).
Phosphorylation of serine 370 and 385 primes PTEN for additional phosphorylation of nearby residues while phosphorylation of serine 380 and threonines 382 and 385 plays a critical role in regulating PTEN stability.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human PTEN when phosphorylated at Ser380, Thr382 and Ser385. Species Crossreactivity: Mouse PTEN. Species sequence homology: Rat (100%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic peptide corresponding to human PTEN that contains serine 380, threonine 382, and serine 385.
