84-1077-54　［取扱停止］PTEN Tumor Suppressor Protein, phosphorylated (Ser385) (MMAC1, Phosphatase and Tensin, TEP1) 10Blots　P9182-14C

特徴

• PTEN (Phosphatase and tensin homolog deleted on chromosome 10) is a ~58kD lipid phosphatase and tumor suppressor protein that is frequently mutated in tumor cells.
• It plays a key role in cell migration, survival and apoptosis by negatively regulating phosphoproteins in the Akt/PI3Kinase pathway and is involved in Toll-like receptor 4 (TLR) and Fc-gamma-mediated macrophage inflammatory responses.
• The non-catalytic regulatory carboxyl terminus of PTEN contains multiple phosphorylation sites including two CK2 sites (serines 370 and 385) and MAGI-2 sites (threonines 382 and 383).
• Phosphorylation of serine 370 and 385 primes PTEN for additional phosphorylation of nearby residues while phosphorylation of serine 380 and threonines 382 and 385 plays a critical role in regulating PTEN stability.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
• Positive Control:HEK293 cells overexpressing WT PTEN
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.