Protein Kinase C, iota (PKCi) is a 76kD member of the atypical group (cPKCs: sensitive to diacylglycerol, phosphotidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion.
Phosphorylation of PKCi plays a key role in protein trafficking.
Phosphorylated PKCi is implicated in high glucose-induced T type II transforming growth factor-beta activity, TNF-a-stimulated NF-kB activation and Bcr/Ablmediated anti-apoptotic effect.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells treated with PMA, a phorbol ester.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human Protein Kinase C, iota and lambda when phosphorylated at Thr555 and Thr563, respectively. Peptide competition suggests crossreaction with Protein Kinase C, zeta [pT560] (83% homologous) and partial crossreaction with Protein Kinase C, betaI [pS642] (58% homologous) and Protein Kinase C, gamma [pT655] (43% homologous).Species Crossreactivity: Mouse Protein Kinase C, iota.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human Protein Kinase C, iota that contains threonine 555. Species Sequence Homology: Mouse Protein Kinase C, iota, Human Protein Kinase C, lambda
