84-1077-52　［取扱停止］Protein Kinase C, eta, phosphorylated (Thr655) (PKCeta) 100ul　P9103-41K

特徴

• Protein Kinase C eta (PKCeta) is an 80kD member of the novel group (nPKCs: sensitive to diacylglycerol, phosphatidylserine and phorbol esters) of the PKC family of serine/threonine kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival, transcriptional regulation and tumor promotion.
• PKC eta has been shown to be involved in the cell cycle as ectopic expression results in elevated expression of cyclins D and E and its association with the cyclinE/cdk2/p21 complex inhibits cdk2 kinase activity, leading to G1 arrest.
• PKC eta is also involved in mediating FGF and PDGF ability to stimulate IL-6 production and PKCm modulation of ERK and JNK signaling pathways.
• The activation loop threonine (threonine 512 in PKC eta) of novel PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1) and is necessary for the autophosphorylation of threonine 655 in the carboxy-terminus of PKC eta, a step that may play a role in mediating PKC eta signaling events.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Positive Control: Jurkat cells treated with PMA, a phorbol ester
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.