PKCy is an 80kD member of the conventional group (cPKCs: sensitive to calcium, diacylglycerol and phorbol esters) of the PKC family of serine/threonine family kinases that are involved in a wide range of physiological processes including mitogenesis, cell survival and transcriptional regulation.
PKCy plays a key role in neuronal signal transduction and is translocated from the nucleus to the cytoplasm upon activation by phorbol ester, where in epithelial cells it has been implicated in regulating intracellular communication.
The activation loop threonine (threonine 514 in PKCy) of conventional PKCs is phosphorylated by phosphoinositide-dependent kinase-1 (PDK1), which is necessary for their autophosphorylation, a critical step in the generation of a catalytically mature enzyme.
Threonine 655 is an autophosphorylation site in the carboxy-terminus of PKCy.
Applications:Suitable for use in Western Blot.
and ELISA Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1ug/mlOptimal dilution to be determined by researcher.
Positive Control:Hela cells treated with PMA, a phorbol ester.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified epitope-specific chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.09% sodium azide.
Specificity：Recognizes human PKCg phosphorylated at Thr655. Species sequence homology: Mouse and rat (100%). Sequence homology: PKCa (69%) and bII (62%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide derived from a region of human PKCy that contains threonine 655. The sequence is conserved in mouse and rat.
