PKCe is a member of the novel protein kinase C family of serine/threonine kinases.
Its activity is calcium-independent and phospholipid-dependent and is expressed at low levels in normal tissue except the brain, where expression is higher.
PKCe expression appears to be induced by interferon-a and its over-expression may play a role in tumorigenesis.
PKCe, through its regulatory domain, can induce immature neurite-like processes via a mechanism that appears to be of importance for neurite outgrowth during neuronal differentiation
Protein kinase C (PKC) mediates a wide range of biological responses, including serotonin release from platelets, histamine release from mast cells, glycogen hydrolysis in the liver, fat synthesis by adipocytes, and many others.
PKC was described as a serine/threonine protein kinase activated by the presence of Ca2+, phospholipids, and sn-1,2 diacylglycerol (Nishizuka, 1986).
In humans, PKC is found as 11 different isoforms which vary in amino acid sequence, structure, subcellular localization, tissue distribution, substrate specificity, and responses to extracellular signals
The PKC isoforms can be grouped based on their requirements for Ca2+ and phospholipids for activation.
The isoforms of group 1 include PKCa, PKCbI, PKCbII, and PKCg.
Group 1 isoforms require both Ca2+ and phospholipids for activation.
The isoforms of group 2 include PKCo, PKCe, PKCh, and PKCq.
Group 2 isoforms require phospholipid for activation, but do not require the presence of Ca2+.
The isoforms of group 3 include PKCz, PKCm, and PKCi.
Group 3 isoforms are not activated by the presence of diacylglycerol or by phorbol esters such as phorbol myristate acetate.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:K562 cells treated with PMA; NIH3T3 cells treated with PDGF
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human and mouse PKCe phosphorylated at Ser729. This antibody may partially cross react with bII [pS660] as determined by peptide competition experiments.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from a region of human PKCe that contains serine 729. Species sequence homology: mouse and rat.
