c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription.
PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP.
The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition.
The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop and serine 338 in the carboxyl terminus.
Phosphorylation of serine 338 plays a key role in stabilizing PKA and activating its substrates and hence mediating its biological functions.
Applications:Suitable for use in ChIP and Western Blot applications.
Other applications not tested.
Recommended Dilution:ChIP: 1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:3T3-L1 adipocytes
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human PKA catalytic b subunit when phosphorylated at Ser338. Species sequence homology: bovine and porcine. Peptide competition data indicate that this antibody cross-reacts with the PKA y subunit (64% homologous) and partially with the a subunit (82% homologous).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human PKA catalytic b subunit that contains serine 338. Species sequence homology: bovine and porcine.
