c-AMP-dependent Protein Kinase (PKA) is a serine/threonine kinase that regulates a number of cellular processes including proliferation, ion transport and gene transcription.
PKA is composed of conserved catalytic subunits and regulatory subunits that dissociate upon activation by cAMP.
The catalytic subunit of PKA contains the activation loop and mediates DNA binding and substrate recognition.
The catalytic subunit is assembled and expressed as an active form and is phosphorylated on threonine 197 by PDK-1 in the activation loop.
Phosphorylation of threonine 197 is critical for PKA maturation, optimal conformation and catalytic activity and plays a key role in mediating its biological functions.
Applications:Suitable for use in Western Blot.
Other applications have not been tested.
Recommended Dilution:Western Blot: 0.1-0.75ug/ml.
At 0.35ug/ml, the dilution provides 100ml working solution, which at 10ml/blot allows 10 blots to be performed.
Optimal dilutions to be determined by the researcher.
Positive Control:Forskolin-treated NIH3T3 cells, or Y-1 mouse adrenal cortical cells.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide.
Specificity：Recognizes human PKA catalytic subunits a and b when phosphorylated at Thr197. Species sequence homology: mouse, rat, bovine, porcine and frog.This antibody exibited a preference for PKA catalytic subunit b in some cell lines tested.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human PKA that contains threonine 197.
