Platelet-derived growth factor receptor (PDGFR) is a transmembrane glycoprotein of 170-185kD which undergoes homo- or heterodimerization into complexes of a and b subunits upon ligand binding, depending on the isoform of PDGF (PDGF-AA, -BB or -AB) that binds.
The phosphorylation of tyrosine residues in the ligand-activated receptor can control multiple signaling events such as actin reorganization, transcription, cell growth, migration and differentiation, and also lead to activation of the Ras → Raf → ERK1&2 pathway.
Tyrosine 742 in PDGFRa is a binding site for PI3-kinase.
Applications:Suitable for use in Western blotting applications.
Other applications not tested.
Recommended Dilution:Western Blot: 0.35-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:NIH3T3 cells +/- PDGF
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes mouse PDGFRa. when phosphorylated at Tyr742 Species sequence homology: human, rat (100%) and frog (83%) PDGFRb (50%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Chemically synthesized phosphopeptide derived from the region of human PDGFRa that contains tyrosine 742.
