Phosphoinositide-specific phospholipase C (PLC) is a family of proteins that hydrolyze phosphatidylinositol 4,5-bisphosphate to generate the second messengers, inositol 1,4,5-trisphosphate (IP3) and diacylglycerol (DAG).
IP3 induces a transient increase in intracellular free Ca++, while DAG is a direct activator of protein kinase C (PKC).
These processes are involved in the control of multiple cellular activities such as cell proliferation, growth and differentiation.
PLCy-1 is a 135kD ubiquitously expressed isozyme that is activated by phosphorylation of tyrosine 783 and interacts with growth factor receptors (EGFR, PDGFR, etc.) and adhesion/cytoskeletal proteins (FAK, paxillin) to influence a multiple number of signaling pathways.
Applications:Suitable for use in Western Blot and Immunocytochemistry.
Optimal dilutions to be determined by the researcher.
Positive Control:NIH3T3 cells +/- PDGF.
Storage and Stability:For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3,1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human PLCy-1 when phosphorylated at Tyr783. Species Crossreactivity: mouse. Species sequence homology: Mouse, rat, cow and frog.
Isotype：IgG
Calc Applications Abbrev：IC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from the region of human PLCy-1 that contains tyrosine 783.
