84-1077-42　［取扱停止］PED/PEA-15, phosphorylated (Ser116) (Phosphoprotein Enriched in Diabetes, Phosphoprotein Enriched in Astrocytes, 15kD) 10Blots　P3139-85

特徴

• PED/PEA-15 (Phosphoprotein Enriched in Diabetes/Phosphoprotein Enriched in Astrocytes-15kD) is a widely expressed 15kD protein comprised of an N-terminal region containing acanonical Death Effector Domain (DED) sequence and a nuclear export signal and a C-terminalregion containing two serine phosphorylation sites.
• PED/PEA-15 has been implicated in theregulation of multiple cellular processes including apoptosis, integrin activation and insulin-sensitive glucose transport in insulin-responsive cells.
• Consistent with this latter function,expression of PED/PEA-15 is increased 2-3-fold in skeletal muscle and adipose cells of patientswith Type II diabetes.
• PED/PEA-15 function is mediated through binding to multiple proteins,including ERK1&2, RSK2, Akt, FADD and Caspase-8, which, in part, modulates their subcellularlocalization and access to potential substrates.
• A major function of PED/PEA-15 may be, therefore, to coordinate inputs from multiple pathways to achieve a unified cellular response
• Phosphorylation of both serine 104 (a Protein Kinase C site) and serine 116 (a substrate of CaMKII and Akt) is required for PED/PEA-15 function.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
• Positive Control:Cos7 cells transfected with PED/PEA-15 cDNA and stimulated with 100 ng/ml PMA; SK-BR-3 cells.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.