Paxillin is a 68kD cytoskeletal adapter protein involved in organization and function of focal adhesions, which are critical to cell adhesion and migration.
This in turn plays a role in a wide variety of processes including embryogenesis, organogenesis, wound repair, inflammation and cancer.
Paxillin contains LD motifs, LIM domains, SH3 and SH2 binding domains that serve as docking sites for cytoskeletal proteins, tyrosine kinases (e.g., FAK, Pyk2, Src), serine/threonine kinases, GTPase activating proteins and other adaptor proteins (e.g., actin, vinculin, Crk).
Tyrosine 118, a Crk binding site of paxillin, is phosphorylated during integrin-mediated cell adhesion.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:NMuMG cells transfected with EGFP-tagged paxillin
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes mouse (93%) Paxillin when phosphorylated at Tyr118. Species sequence homology: human (100%) and chicken (87%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo
Immunogen：Synthetic peptide corresponding to human paxillin that contains tyrosine 118.
