p21-activated kinase (PAK) is actually a family of serine/threonine protein kinases, members of which are activated by small molecular weight GTPases.
The three most common isoforms arePAK1, PAK2, and PAK3 (also known as a-PAK, y-PAK, and b-PAK, respectively).
These kinases contain numerous regulatory elements that trigger diverse signaling processes such as those initiated by activated GTPases, interaction with Src homology 3 (SH3) domains, and caspasemediated proteolytic cleavage.
Autophosphorylation of serine 141 in the SH2 domain of PAK2 (serine 144 and serine 139 for PAK1 and PAK3, respectively) results in initial activation of PAK, leading to the phosphorylation of threonine 423 and full activation.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human PAK1 (46%), PAK2, and PAK3 (46%) when phosphorylated at Ser144/Ser141/Ser139, respectively. Other species have not been tested, but are expected to react.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human PAK2 that contains serine 141. Species sequence homology: rat and rabbit.
