Although the function of k-opioid receptors is unclear, recent evidence indicates that activation of the k-opioid receptor opposes a variety of mu-opioid receptor mediated actions throughout the brain and spinal cord.
In general, opioids modulate numerous central and peripheral processes, including pain perception, neuroendocrine secretion and the immune response.
The opioid signal is transduced from receptors through G proteins to various different effectors.
Subsequent to G protein activation, several effectors are known to orchestrate the opioid signal.
For example, activation of opioid receptors increases phosphatidylinositol turnover, activates K+ channels and reduces adenylyl cyclase and Ca++ channel activities.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, aliquot and store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by peptide affinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol. Also available BSA/carrier free, see O7030-34.
Specificity：Recognizes mouse opioid receptor. Species Crossreactivity: rat. Species sequence homology: human 93%.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Mo Rt
Immunogen：Synthetic peptide derived from the internal region of the mouse k-opioid receptor.
