Nuclear factor-kB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses.
All members share a conserved amino-terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy-terminal transactivation domain.
Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL-1b and TNF-a as mediated by IkB kinase and p38 MAPK.
Serine 529 is located in a negatively charged region(amino acids 422-540) that is phosphorylated in response to phorbol myristate acetate plus calcium ionophore activation.
Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:Jurkat cells treated with PMA and Ca2+ ionophore.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human NFkB when phosphorylated at Ser529. Species sequence homology: mouse NFkB (91%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human NFkB that contains serine 529.
