Smooth Muscle Myosin Light Chain Kinase (MLCK) is a multifunctional regulatory protein of smooth muscle contraction (SMC) and a key element in ligand-mediated endothelial cell gap formation and vascular permeability, motility and morphology.
Smooth muscle MLCK exists in at least two isoforms, short (~150kD) and long (210kD) which are identical except for an extended amino terminus with two additional putative actin-binding motifs in the long isoform
MLCK is phosphorylated by several kinases including protein kinase A (PKA) and mediates its function by phosphorylating 20kD myosin light chain (MLC20).
Phosphorylation of MLCKinhibits the actin-activated ATPase of myosin II by reducing its affinity for actin.
MLCK possesses a counter-balancing role in vascular regulation, by mediating vasoconstriction via directaction on SMCs and vasodilation via action on endothelial cells (ECs).
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Controls:KCl-treated rabbit muscle extracts; COS cells over-expressing rabbit smooth muscle short MLCK, phosphorylated in vitro by PKA.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in Dulbecco’s PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes MLCK when phosphorylated at Ser1760. Species Crossreactivity: rabbit, mouse and bovine. Species Sequence Homology: human,100%.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Bo Hu Mo Rb
Immunogen：Synthetic phosphopeptide derived from the region of human myosin light chain kinase (MLCK) that contains serine 1760.
