Mitogen-Activated Protein Kinase Kinase 7 (MEK7 or MKK7) is a 48kD member of a tyrosine/threonine protein kinase family that activates the c-Jun NH2-terminal kinases (JNK), which is part of the inflammation/stress signaling pathway.
Phosphorylation of MEK7 by MEKK1 on serine 271 and threonine 275 in the catalytic domain activates the protein and enables MEK7 to phosphorylate JNK.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilutions:Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:GST-tagged fusion protein expressing MEK7
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size:10Blots
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.3, 1mg/ml BSA (IgG, protease free), 0.05% sodium azide
Specificity:Recognizes human MEK7 when phosphorylated at Ser271 and Thr275. Does not react with other MEK isoforms.
Isotype:IgG
Calc Applications Abbrev:WB
Calc Crossreactivity:Hu
Immunogen:Synthetic phosphopeptide derived from a region of human MEK7 that contains serine 271 and threonine 275. Species Sequence Homology: mouse, 100%