84-1077-30　［取扱停止］MEK1, phosphorylated (Thr386) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-Activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1) 10Blots　M2865-11F

特徴

• Microtubule-associated protein kinase kinase 1 (MEK1 or MAPKK1) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1&2/MAPK enzymes by phosphorylatingboth residues within the threonine – glutamic acid – tyrosine (TEY) motif in the activation loop.
• MEK1&2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
• Threonine 386 of MEK1 is phosphorylated by ERK2, which serves as a negative feedback loop by suppressing activation of MEK2.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
• Positive Control:NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.