Mitogen-activated protein kinase kinase 1 (MEK1 or MAPKK1, 43.5kD) is a member of a family of tyrosine/threonine protein kinases that activate the ERK1&2/MAPK enzymes by phosphorylating both residues within the threonine-glutamic acid-tyrosine (TEY) motif in the activation loop.
MEK1&2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
Threonine 292 of MEK1 is phosphorylated by ERK1&2, which serves as a negative feedback loop by suppressing activation of MEK1.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:NIH3T3 +/- PDGF; recombinant wild-type vs.
mutant (T292A) MEK1 treated with ERK to induce phosphorylation.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human and mouse MEK1 when phosphorylated at Thr292.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from a region of human MEK1 that contains threonine 292.
