84-1077-28　［取扱停止］MEK1, MEK2, phosphorylated (Ser222) (MAPK/ERK Kinase 1, MEK 1, Dual Specificity Mitogen-activated Protein Kinase Kinase 1, MAP Kinase Kinase 1, MAP2K1, MAPKK 1, MKK1, PRKMK1, ERK Activator Kinase 1 / MAPK/ERK Kinase 2, Dual Specificity Mitogen-activated Protein Kinase Kinase 2, MAP Kinase Kinase 2, MAP2K2, MAPKK 2, MKK2, PRKMK2, ERK Activator Kinase 2) 10Blots　M2865-10G

特徴

• Microtubule-associated protein kinase kinase 1&2 (MEK1&2 or MAPKK1&2) are members of a tyrosine/threonine protein kinase family that activate the ERK1&2/MAPK enzymes by phosphorylating both residues within the threonine – glutamic acid – tyrosine (TEY) motif in theactivation loop.
• MEK1&2 are also activated by dual-phosphorylation, which occurs on serines 218 and 222, in the activation loop of the MEKs.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
• Positive Control:NIH3T3 cells +/- PDGF, A431 +/- EGF, PC12 +/- NGF
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.