84-1077-26　［取扱停止］MAPKAP Kinase 2, phosphorylated (Thr334) (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2) 10Blots　M2355-13A

特徴

• Mitogen activated protein kinase-activated protein kinase-2 (MAPKAPK-2), expressed as alpha(~49kD) and beta (~60kD) isoforms, is phosphorylated by p38 MAPK and mediates multiple p38 MAPK-dependent cellular responses, including inflammation.
• MAPKAPK-2 is implicated in several disorders including brain ischemic injury and heart failure, and has shown to be important in regulating stress resistance and the production of tumor necrosis factor alpha (TNF-a).
• In unstimulated cells, MAPKAPK-2 is located in the nucleus where it contributes to CREB phosphorylation.
• Upon stimulation it translocates to the cytoplasm and phosphorylates andactivates tuberin and HSP27.
• MAPKAPK-2 is phosphorylated in the kinase domain at threonine222 (in the activation loop) and serine 272, and in the C-terminus at threonines 334 and 338
• Threonine 334 is a major regulatory site, as once phosphorylated it induces conformationalchanges which result in the activation of the kinase.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
• Positive Control:HeLa cells treated with TNF-a or anisomycin; NIH-3T3 cells treated with anisomycin.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.