The Lin-11, Is1-1 and Mec-3 kinases 1 and 2 (LIMK1 [72kD] and LIMK2 [65kD]) are serine/threonine kinases that are involved in actin cytoskeletal reorganization, cell growthregulation and tumor supression.
LIMK1/2 are activated by various extracellular stimuli including stromal cell-derived factor-1alpha (SDF-1alpha) and insulin, through activation of small GTPases (Rho, Rac-1 and Cdc-42) and their downstream kinases, ROCK and PAK1.
ROCK directly phosphorylates LIMK1 at threonine 508 and LIMK2 at threonine 505.
LIMK1 plays a role in Racinduced lamellipodia formation, whereas LIMK2 plays a role in Cdc42-induced filipodial formation and Rho-induced stress fiber formation.
LIMK1/2 negatively regulate cofilin activity by phosphorylating cofilin at serine 3 and hindering it from associating with actin.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Controls:Jurkat cells treated with SDF-1alpha; A431 cells treated with EGF.
Storage and Stability:May be stored at 4°C for short-term only.
For long-term storage, store at -20°C.
Aliquots are stable for at least 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by epitope-specific chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.09% sodium azide, 50% glycerol.
Specificity：Recognizes Human LIMK1 when phosphorylated at tyrosine 507 and threonine 508 (tyrosine 504 and threonine 505 in LIMK2).. Species sequence homology: Mouse, rat and frog.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from the region of human LIMK1 that contains tyrosine 507 and threonine 508 (tyrosine 504 and threonine 505 in LIMK2).
