Linker for activation of T cells (LAT) is a 36kD transmembrane adapter protein that is tyrosine-phosphorylated following T-cell receptor (TCR) stimulation by ZAP-70 and Syk.
Four distal tyrosine residues (Y132, Y171, Y191 and Y226 in human LAT) are crucial for its activity and subsequent signaling to downstream molecules.
Tyrosine 132 mediates LAT binding to PLC-y-1 and is necessary for TCR-mediated calcium mobilization and activation of ERK and NFAT proteins.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-1ug/mlOptimal dilutions to be determined by the researcher.
Positive Control:Jurkat E6.1 cells
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after recipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide.
Specificity：Recognizes human LAT when phosphorylated at Tyr132.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide derived from a region of human LAT that contains tyrosine132. Species sequence homology: mouse (85%) and rat (77%).
