c-Jun N-terminal Kinase (JNK) is a Stress Activated Protein Kinase/Map Kinase (SAPK/MAPK) represented by three isoforms, JNK1 (~49kD), JNK2 (~55kD) and JNK3 (~54kD).
JNK is dually phosphorylated and therefore fully activated by MEK4 and MEK7 on threonine 183 and tyrosine 185 within the activation loop.
The JNK and p38 pathways function to modulate cellcycle, apoptotic and transcriptional responses to stress.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human, mouse, and rat JNK1&2 when phosphorylated at Thr183, Tyr185. Other species of JNK1&2 have not been tested, and JNK3 (found primarily only in neuronal cell lines) has not been detected.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic phosphopeptide corresponding to human JNK1&2 contains threonine 183 and tyrosine 185. Species sequence homology: mouse, rat, chick, nematode, and fruit fly, and in JNK3.
