84-1077-17　［取扱停止］JAK1, phosphorylated (Tyr1022, Tyr1023) (Janus Kinase 1) 10Blots　J0900-20B

特徴

• Janus Kinase 1 (JAK1) is a 130kD tyrosine kinase involved in cytoplasmic signal transduction.
• Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1022 and 1023 in the kinase activation loop.
• Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus.
• Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes, which can lead to various responses such as cell proliferation, cell survival and differentiation.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Positive Controls:3T3-L1 adipocytes +/- Leukemia Inhibitory Factor (LIF).
• Storage and Stability:For long-term storage, aliquot and store at -20°C.
• Aliquots are stable for at least 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.