Insulin receptor substrates (IRS) are responsible for several insulin related activities, such as glucose homeostasis, cell growth, cell transformation, apoptosis and insulin signal transduction.
Serine/threonine phosphorylation of IRS-1 has been demonstrated to be a negative regulator of insulin signaling and is responsible for its degradation, although IRS-1 degradation pathways are not well understood.
IRS-1 has also been shown to be constitutively activated in cancers such as breast cancer, Wilm"e;s tumors, and adrenal cortical carcinomas, thus making IRS-1 phosphorylation and subsequent degradation an attractive therapeutic option.
To date there have been four subtypes identified: IRS-1,2,3, and 4, with IRS-1 being widely expressed.
Applications:Suitable for use in Flow Cytometry, Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry (Paraffin): 1:10-1:100Flow Cytometry: 3-5ug/million cellsOptimal dilutions to be determined by the researcher.
Positive Control:CHO-T cells transiently transfected with human IRS-1
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for 12 months after receipt.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.3, 1mg/ml BSA, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human IRS-1 when phosphorylated at Tyr612. Species Crossreactivity: mouse
Isotype：IgG
Calc Applications Abbrev：E FC IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：Synthetic phosphopeptide derived from a region of human IRS-1 that contains tyrosine 612. Species sequence homology: rat, chicken
