84-1077-09　［取扱停止］Integrin, beta3, phosphorylated (Tyr785) (Platelet Glycoprotein IIIa) 10Blots　I7661-42H

特徴

• Specificity: Integrin 3, also known as CD61, is a 130kD transmembrane glycoprotein that binds noncovalently in complexes with integrin alpha subunits (alphaIIb, alphav) to form the functional receptor that binds to specific extracellular matrix proteins (e.g., fibronectin, vitronectin, etc.).
• Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis and prevention of programmed cell death.
• They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth and metastasis.
• These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells.
• The clustering of integrin receptors on the cell surface and their binding to the extracellular matrix leads to the formation of focal contacts and the activation of various signal transduction pathways.
• Phosphorylation of tyrosine 785 on Integrin beta3 is essential for Shc and Grb2 binding and promotes cell migration.
• Tyr785 is commonly referred to as Tyr759, the corresponding site in the chicken Integrin beta3 protein.
• Applications:Suitable for use in ELISA and Western Blotting.
• Other applications not tested.
• Recommended Dilution:Western Blotting: 1:1000-1:2000Optimal dilutions to be determined by the researcher.
• Positive Control:K562 cells transfected with human Integrin av and wild-type (WT) or mutant (Y785F) humanIntegrin b3, and analysed by immunoprecipitation with 1A2 (human Integrin b3) mAb.
• Storage and Stability:May be stored at 4°C for short-term only.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Aliquots are stable for at least 12 months.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.