Biological actions of insulin are mediated by the insulin receptor (IR), a receptor tyrosine kinase that regulates multiple signaling pathways through activation of a series of phosphorylation cascades.
The IR is a heterotetrameric protein consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain.
Insulin binding to the extracellular domain leads to autophosphorylation and internalization of the receptor, and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated.
Tyrosine 972 is in the juxtamembrane Asn-Pro-Glu-Tyr (NPEY) motif.
Phosphorylation of IR tyrosine 972 is required for the binding and/or phosphorylation of the adapter protein Shc, the PTB domain, IRS-1, PI3 kinase, and the Suppressor of Cytokine Signaling (SOCS).
Applications:Suitable for use in Western Blot and ELISA.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Positive Control:CHO-T cells transfected with a vector encoding the human insulin receptor and stimulated with insulin, and 3T3-L1 adipocytes +/- insulin stimulation
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human IR when phosphorylated at Tyr972. Species sequence homology: Rat, mouse (100%), IGF1R pY950 (75%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to the human insulin receptor that contains tyrosine 972, as numbered according to Ebina, et al. (tyrosine 960 according to Ullrich, et al.). Species sequence homology: mouse and rat.
