Biological actions of insulin and IGF1 are mediated by their respective cell surface receptors, both of which are receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades.
The IR and IGF1R are heterotetrameric proteins consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain.
Insulin/IGF1 binding to the extracellular domain leads to autophosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, which allows appropriate substrates to be phosphorylated.
These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains.
These differences allow insulin and IGF-1 to regulate different physiological functions through receptors that share a very similar structure.
Phosphorylation sites that are unique to each receptor presumably play a key role in these signaling differences.
The catalytic loops within the tyrosine kinase domains of the IR/IGF1R contain a three tyrosine motif.
It is generally believed that autophosphorylation within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 for the IR or 1135 for the IGF1R), followed by phosphorylation at the first tyrosine (1158 or 1131), then the last (1163 or 1136), upon which the IR or IGF1R becomes fully active.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:CHO-T cells transfected with a vector encoding the human insulin receptor and stimulated withinsulin, and 3T3-L1 adipocytes +/- insulin stimulation.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.3, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human and mouse IR/IGF1R when phosphorylated at Tyr1131, Tyr1135, Tyr1136 (IGFR) or Tyr1158, Tyr1162, Tyr1163 (IR). Species sequence homology: Rat (100%).
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to IR/IGF1R that contains tyrosines 1158, 1162 and 1163 of the human insulin receptor (IR). The corresponding residues in the IGF1R are 1131, 1135 and 1136.
