Biological actions of insulin and IGF-1 are mediated by their respective cell surface receptor tyrosine kinases that regulate multiple signaling pathways through activation of a series of phosphorylation cascades.
The IR and IGF-1R are heterotetrameric proteins consisting of two ligand-binding a subunits and two b subunits that each contain a tyrosine kinase domain.
Insulin/IGF-1 binding to the extracellular domain leads to auto-phosphorylation of the receptor and activation of the intrinsic tyrosine kinase activity, leading to tyrosine phosphorylation of downstream target proteins.
These two receptors differ in sequence in regions that confer specificity for the designated ligand as well as in certain intracellular signaling domains, resulting in significant differences in the functional consequences of activation of each receptor
The catalytic loops within the tyrosine kinase domains of the IR/IGF1R contain a three tyrosine motif corresponding to tyrosines 1158, 1162 and 1163 (for the IR) and 1131, 1135 and 1136 (for the IGF1R).
It is generally believed that autophosphorylation within the activation loop proceeds in a processive manner initiating at the second tyrosine (1162 or 1135), followed by phosphorylation at the first tyrosine (1158 or 1131), then the last (1163 or 1136), upon which the IR or IGF1R becomes fully active.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000 Optimal dilutions to be determined by the researcher.
Positive Control:CHO-T cells transfected with a vector containing insulin receptor and stimulated with 100 nM insulin for 10 minutes at 37oC.
Storage and Stability:May be stored at 4°C for short-term only.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Aliquots are stable for at least 12 months.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
仕様
Size：10Blots
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS (without Mg2+ and Ca2+), 1mg/ml BSA (IgG, protease free), pH 7.2, 0.05% sodium azide, 50% glycerol.
Specificity：Recognizes human IR/IGF1R when phosphorylated at Tyr1135 and Tyr1136 (IGFR), or Tyr1162 and Tyr1163 (IR). Species sequence homology: mouse and rat (100%).
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：Synthetic phosphopeptide corresponding to IR/IGF1R that contains tyrosines 1162 and 1163 of the human insulin receptor (IR). The corresponding residues in the IGF1R are 1135 and 1136. Species sequence homology: mouse and rat for both the IR and IGF1R.
